In addition, the center supports library preparation from the cDNA generated using microfluidic-based systems such as C1 Fluidigm systems.
Single-Cell Analysis Services
The following services are supported using the 10x Genomics Chromium system:
- single-cell or single nucleus RNA-seq
- single-cell ATAC-seq
- single-cell CNV (Copy Number Variation)
- single-cell immune profiling (with antigen specificity and cell surface proteins)
Before each single-cell experiment, the center requests a project planning meeting including the PI, experimentalists (lab technician, postdocs or graduate students), core staff, and bioinformatician. In this meeting, the key experiment logistics are discussed, including cell quality assessment, experiment scheduling, expectation, analysis plan and data dissemination. The center also encourages a “dry run” before an experiment to assess the quality and quantity of the single-cell suspension.