Faculty

Pratibha Singh, PhD, MSC

Assistant Professor of Pediatrics

Phone (317) 278-9944

Address

Wells R4
PNEB
IN
Indianapolis, IN

Key Publications

Pratibha Singh, Yongxue Yao, Abigail Weliver, Hal E. Broxmeyer, Soon-Cheol Hong, Cheong-Hee Chang., Vaccinia virus infection modulates the hematopoietic cell compartments in the bone marrow. Stem Cells. 2008 Apr; 26(4):1009-16.

Jonathan Hoggatt, Pratibha Singh, Janardhan Sampath, and Louis M. Pelus, Prostaglandin E2enhances hematopoietic stem cell homing, survival, and proliferation. Blood. 2009 May 28; 113 (22):5444-55.

Pratibha Singh, Jonathan Hoggatt, Peirong Hu, Jennifer M. Speth, Seiji Fukuda, Richard M. Breyer, and Louis M. Pelus, Blockade of prostaglandin E2signaling through EP1 and EP3 receptors attenuates Flt3L-dependent dendritic cell development from hematopoietic progenitor cells. Blood. 2012 Feb 16; 119(7):1671-1682.

Pratibha Singh, Peirong Hu, Jonathan Hoggatt, Akira Moh and Louis M. Pelus,

Expansion of bone marrow neutrophils following G-CSF administration in mice results in osteolineage cell apoptosis and mobilization of hematopoietic stem and progenitor cells.

Leukemia. 2012 Nov;26(11):2375-83.

Jonathan Hoggatt, Khalid S Mohammad†,Pratibha Singh†, Hoggatt AF, Chitteti BR, Speth JM, Hu P, Poteat BA, Stilger KN, Ferraro F, Silberstein L, Wong FK, Farag SS, Czader M, Milne GL, Breyer RM, Serezani CH, Scadden DT, Guise TA, Srour EF, Pelus LM., Differential stem- and Progenitor-cell trafficking by prostaglandin E2.Nature. 2013 Mar 21;495(7441):365-9. (†contributed equally).

Pratibha Singh, Louis M. Pelus. IFN-1 Bid crosstalk: foe or friend to stem cells. Stem Cell Investig. 2017 Feb 27; 4:18. doi: 10.21037/sci.2017.02.06. eCollection 2017.

Pratibha Singh, Jonathan Hoggatt, Malgorzata M. Kamocka, Khalid S. Mohammad, Mary R. Saunders, Hongge Li, Jennifer Speth, Nadia Carlesso, Theresa A Guise and Louis M. Pelus. Neuropeptide Y regulates a vascular gateway for hematopoietic stem and progenitor cells. J Clin Invest.2017;127(12):4527-4540.

Jonathan Hoggatt, Pratibha Singh, Tiffany A. Tate, Bin-Kaun Chou, Shruti R. Datari, Seiji Fukuda, Liqiong Liu, Peter V. Kharchenko, Amir Schajnovitz, Ninib Baryawno, Francois E. Mercier, Joseph Boyer, Jason Gardner, Dwight M. Marrow, David T. Scadden. Rapid Mobilization Reveals a Highly Engraftable Hematopoietic Stem Cell. Cell. 2018 Jan 11;172(1-2):191-204.

Pratibha Singh, Seiji Fukuda, Liqiong Liu, Brahmananda Reddy Chitteti, Louis M. Pelus. Survivin Is Required for Mouse and Human Bone Marrow Mesenchymal Stromal Cell Function. Stem Cells. 2018 Jan;36(1):123-129.

Titles & Appointments

Assistant Professor of Pediatrics

Education

2006 PhD Sanjay Gandhi Postgraduate Institute of Medical Sciences

1998 MSC Dr. Ram Manohar Lohia Avadh University

1996 BSC Mahatma Gandhi College
Research

My research focuses on understanding mechanisms that regulate normal and stressed hematopoietic stem and progenitor proliferation, differentiation and survival, and the role played by bone marrow niche support cells in hematopoietic stem cell function.

Current clinical hematopoietic transplant (HCT) relies on moving HSPC from bone marrow (BM) to the circulation, a process called peripheral blood stem cell mobilization (PBSCM), with G-CSF (Neupogen) being the primary drug used clinically for PBSCM. However, little is known about the mechanisms governing HSPC egress. Understanding this process has major relevance for clinical HCT. Poor homing and inadequate expansion, survival and differentiation of transplanted HSPC can limit transplant efficacy. Understansing hematopoietic stem cell regulation will allow us to develop improved stratiegies for hematopoietic stem cell based therapies, and facilitate recovery of hematopoieisis following stress and injury such as chemo/radio therapy or acute radiation exposure. My research interests include identifying mechanisms of action of Neuropeptide Y on hematopoietic cells and on the function of stromal niche support cells and in regulation of hematopoietic stem and progenitor cells trafficking. I am currently exploring the role of sinusoidal endothelial cells in hematopoietic stem cell maintenance and trafficking and have identified an unappreciated role for DPP IV- Neuropeptide Y axis in regulation of endothelial cell barrier permeability and hematopoietic stem cell trafficking. I am also investigating the role of Survivin in regulating mesenchymal stem cell functions and I have identified a previously unknown role for Survivin in the mesenchymal stem cell survival/proliferation and maintenance of hematopoietic stem cell niche. My emerging interest is to investigate the role of stromal cell populations in regulation of hematopoiesis following stress, particularly in niche regeneration after myeloablative injury such as radiation exposure.
Publications

Aging negatively impacts the ability of megakaryocytes to stimulate osteoblast proliferation and bone mass.
Maupin KA; Himes ER; Plett AP; Chua HL; Singh P; Ghosh J; Mohamad SF; Abeysekera I; Fisher A; Sampson C; Hong JM; Childress P; Alvarez M; Srour EF; Bruzzaniti A; Pelus LM; Orschell CM; Kacena MA; Bone 2019 Jul 9

Survivin Is Required for Mouse and Human Bone Marrow Mesenchymal Stromal Cell Function.
Singh P; Fukuda S; Liu L; Chitteti BR; Pelus LM; Stem cells (Dayton, Ohio) 2017 Nov 8

Neuropeptide Y regulates a vascular gateway for hematopoietic stem and progenitor cells.
Singh P; Hoggatt J; Kamocka MM; Mohammad KS; Saunders MR; Li H; Speth J; Carlesso N; Guise TA; Pelus LM; The Journal of clinical investigation 2017 Nov 13

IFN-1 Bid crosstalk: foe or friend to stem cells.
Singh P; Pelus LM; Stem cell investigation 2017 Feb 27

Rapid Mobilization Reveals a Highly Engraftable Hematopoietic Stem Cell.
Hoggatt J; Singh P; Tate TA; Chou BK; Datari SR; Fukuda S; Liu L; Kharchenko PV; Schajnovitz A; Baryawno N; Mercier FE; Boyer J; Gardner J; Morrow DM; Scadden DT; Pelus LM; Cell 2017 Dec 7

Internal Tandem Duplication in FLT3 Attenuates Proliferation and Regulates Resistance to the FLT3 Inhibitor AC220 by Modulating p21Cdkn1a and Pbx1 in Hematopoietic Cells.
Abe M; Pelus LM; Singh P; Hirade T; Onishi C; Purevsuren J; Taketani T; Yamaguchi S; Fukuda S; PloS one 2016 Jul 7

Survivin modulates genes with divergent molecular functions and regulates proliferation of hematopoietic stem cells through Evi-1.
Fukuda S; Hoggatt J; Singh P; Abe M; Speth JM; Hu P; Conway EM; Nucifora G; Yamaguchi S; Pelus LM; Leukemia 2014 Jun 6

Prostaglandin E2 enhances long-term repopulation but does not permanently alter inherent stem cell competitiveness.
Hoggatt J; Mohammad KS; Singh P; Pelus LM; Blood 2013 Sep 18

Pharmacologic increase in HIF1a enhances hematopoietic stem and progenitor homing and engraftment.
Speth JM; Hoggatt J; Singh P; Pelus LM; Blood 2013 Oct 28

Differential stem- and progenitor-cell trafficking by prostaglandin E2.
Hoggatt J; Mohammad KS; Singh P; Hoggatt AF; Chitteti BR; Speth JM; Hu P; Poteat BA; Stilger KN; Ferraro F; Silberstein L; Wong FK; Farag SS; Czader M; Milne GL; Breyer RM; Serezani CH; Scadden DT; Guise TA; Srour EF; Pelus LM; Nature 2013 Mar 13

Recovery from hematopoietic injury by modulating prostaglandin E(2) signaling post-irradiation.
Hoggatt J; Singh P; Stilger KN; Plett PA; Sampson CH; Chua HL; Orschell CM; Pelus LM; Blood cells, molecules & diseases 2012 Nov 30

Expansion of bone marrow neutrophils following G-CSF administration in mice results in osteolineage cell apoptosis and mobilization of hematopoietic stem and progenitor cells.
Singh P; Hu P; Hoggatt J; Moh A; Pelus LM; Leukemia 2012 Apr 30

Blockade of prostaglandin E2 signaling through EP1 and EP3 receptors attenuates Flt3L-dependent dendritic cell development from hematopoietic progenitor cells.
Singh P; Hoggatt J; Hu P; Speth JM; Fukuda S; Breyer RM; Pelus LM; Blood 2011 Nov 22

Pulse exposure of haematopoietic grafts to prostaglandin E2 in vitro facilitates engraftment and recovery.
Pelus LM; Hoggatt J; Singh P; Cell proliferation 2011 Apr

Survivin mediates aberrant hematopoietic progenitor cell proliferation and acute leukemia in mice induced by internal tandem duplication of Flt3.
Fukuda S; Singh P; Moh A; Abe M; Conway EM; Boswell HS; Yamaguchi S; Fu XY; Pelus LM; Blood 2009 May 1

Prostaglandin E2 enhances hematopoietic stem cell homing, survival, and proliferation.
Hoggatt J; Singh P; Sampath J; Pelus LM; Blood 2009 Mar 26

Vaccinia virus infection modulates the hematopoietic cell compartments in the bone marrow.
Singh P; Yao Y; Weliver A; Broxmeyer HE; Hong SC; Chang CH; Stem cells (Dayton, Ohio) 2008 Feb 7

Vaccinia virus infection induces dendritic cell maturation but inhibits antigen presentation by MHC class II.
Yao Y; Li P; Singh P; Thiele AT; Wilkes DS; Renukaradhya GJ; Brutkiewicz RR; Travers JB; Luker GD; Hong SC; Blum JS; Chang CH; Cellular immunology 2007 Aug 2

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